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Immune cell (peripheral, mucosal and brain) isolation, purification and culture/stimulation in vitro (inflammatory or mitogen challenge, TLR ligand stimulation)

Cytokine Assays (ELISA) on cell culture supernatants, colostrum and milk

Pig immunoglobulin dosage (serum, colostrum and milk)

Membrane and /or intracellular staining and multi-colour flow cytometry (acquisition – analysis)
Mice, sheep, human and pig experimentation

Research topics and scientific objectives

My research activity deals with the consequences of perinatal nutrition on the development and responsiveness of intestinal immune system in the offspring (nutritional imprinting) as well as on the adult immune system, notably its susceptibility to inflammation (peripheral, infiltrating brain immune cells), in interaction with adult nutritional environment.

Education and main positions held

From 2017 Research associate, Institute NuMecan, Nutrition Gut Brain team, St-Gilles, France

From 2012 Research associate, INRA, Food and Digestive, Nervous and Behavioural Adaptations Unit (UR ADNC), St-Gilles, France

2010-2012 Research associate, INRA, Livestock Production, Animal and Human Nutrition Unit (UMR SENAH), St-Gilles, France

2008-2009 Postdoctoral fellow (cellular & molecular immunology), Dr F. Laurent, Control and immunology of gut diseases in the newborn team, UMR Infectiology and Public health, INRA Val de Loire, Nouzilly, France

2004-2007 Postdoctoral fellow (cellular immunology & proteomics), Dr A.P. Mountford, Schistosomiasis Research Group, Biology Department, University of York, United Kingdom

2004 PhD in Biology (specialty immunology): “Role of myeloid cells in type 1 diabetes pathogenesis. Deficiencies of inhibitory macrophage differentiation, maturation and function in NOD mice”, Cellular & Molecular Immuno-Endocrinology Unit, INRA/National Veterinary School/University of Nantes, France

2000 Master of Research in Cellular Biology, University of Nantes, France
1999 Master of Sciences in Cellular Biology and Physiology, University of Nantes, France
1998 Bachelor of Science in Biology, University of Nantes, France

Current projects

FOSGUT (Meiji Tereos-Syral research contract 2013-2016) – Maternal diet rich in fructo-oligosaccharides and metabolic and immune responses of the progeny to a high-fat diet
CLIMAPIG (Lactalis 2014-2017) – Interaction matrice lipidique des formules infantiles, et biologie intestinale, conséquences sur le métabolisme glucidique de l’adulte
NeoDC (ANR 2012-2013) – Mucosal dendritic cells: a major target to strengthen the health of the young animal

Selected publications

The Cheese Matrix Modulates the Immunomodulatory Properties of Propionibacterium freudenreichii CIRM-BIA 129 in Healthy Piglets.
Rabah H, Ferret-Bernard S, Huang S, Le Normand L, Cousin FJ, Gaucher F, Jeantet R, Boudry G, Jan G.
Frontiers in Microbiology 2018 Oct; 9:2584

Perinatal short-chain fructooligosaccharides program intestinal microbiota and improve enteroinsular axis function and inflammatory status in high-fat diet-fed adult pigs.
C. Le Bourgot, S. Ferret-Bernard, E. Apper, B. Taminiau, A. Cahu, L. Le Normand, F. Respondek, I. Le Huërou-Luron, S. Blat.
FASEB Journal 2019 Jul; 33

Maternal short chain fructo-oligosaccharide supplementation increases intestinal cytokine secretion, goblet cell number, butyrate concentration and Lawsonia intracellularis humoral vaccine response in weaned pigs.
C. Le Bourgot, L. Le Normand, M. Formal, F. Respondek, S. Blat, E. Apper, S. Ferret-Bernard, I. Le Huërou-Luron.
British Journal of Nutrition 2017 Jan; 117(1):83

Short-chain fructooligosaccharide supplementation during gestation and lactation or after weaning differentially impacts pig growth and IgA response to influenza vaccination.
C. Le Bourgot, S. Ferret-Bernard, S. Blat, E. Apper, I. Le Huërou-Luron.
Journal of Functional Foods 2016 Jun; 24:307

Maternal 18:3n-3 favors piglet intestinal passage of LPS and promotes intestinal anti-inflammatory response to this bacterial ligand.
C. Desaldeleer, S. Ferret-Bernard, F. de Quelen, L. Le Normand, C. Perrier, G. Savary, V. Romé, C. Michel, J. Mourot, I. Le Huërou-Luron, G. Boudry.
The Journal of Nutritional Biochemistry 2014 Oct; 25(10):1090

HAL Publications

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